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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Exp Neurol. 2008 Oct 7;215(1):60–68. doi: 10.1016/j.expneurol.2008.09.016

Fig. 3. Comparison of [C57BL/6JxDBA/2J] F1 hybrid mice with parental strains in 10% flurothyl-induced generalized seizure thresholds over the 8-trials of the induction phase and following incubation and retest.

Fig. 3

The latency to loss of postural control (generalized seizure threshold (GST)) throughout the full regimen of the flurothyl kindling model in C57BL/6J (n=28), DBA/2J (n=28), and [C57BL/6J x DBA/2J] F1 hybrid (n=28) mice (data from C57BL/6J and DBA/2J mice used in Figure 1 are re-plotted for comparison). The first exposure to flurothyl (10%) on trial 1 serves as an indicator of the baseline GST for each strain. C57BL/6J mice (black circles) had the highest GST (most resistant), while the DBA/2J mice (red squares) had the lowest GST (most susceptible). The [C57BL/6J x DBA/2J] F1 hybrid (blue squares with open white circles) mice had an intermediate GST. Successive daily exposures to flurothyl for 8-days resulted in a decreased latency to loss of postural control in both C57BL/6J and in [C57BL/6J x DBA/2J] F1 hybrid mice, which was not seen in DBA/2J mice. Throughout the entire 8 trial flurothyl kindling procedure, C57BL/6J, DBA/2J, and [C57BL/6J x DBA/2J] F1 hybrid mice all expressed clonic-forebrain seizures. Following a 28-day rest period, mice were again exposed to flurothyl. On this retest, C57BL/6J (filled circles) and [C57BL/6J x DBA/2J] F1 hybrid mice had the same latency to loss of postural control as they had had on trial 8 of the induction-phase. However, the GST of DBA/2J mice significantly decreased upon retest (P < 0.02). No DBA/2J, C57BL/6J, or [C57BL/6J x DBA/2J] F1 hybrid mice had lethal forebrain→brainstem seizures during the induction-phase. @ denotes significance of difference from DBA/2J (P < 0.03); $ denotes significance of difference from [C57BL/6J x DBA/2J] F1 hybrid (P < 0.03); # denotes significance of difference from DBA/2J (P < 0.0002); + denotes significance of difference from DBA/2J - trial 8 of the induction-phase (P < 0.02).