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Time-course analyses of cyclic nucleotide phosphodiesterase (CPDase) activities of PF0027 and ligT proteins. (A) Representative time-course analysis of CPDase activity on 8 M urea–20% PAGE gel. The reaction was carried out with 125 ng/mL purified PF0027 protein at 60°C using the FAM-R8 probe digested by RNase T1. Quantitative plots of the time-course analysis for (B) PF0027 protein and (C) ligT protein are shown. Data shown are mean ± SEM of three experiments.
