Fig 7.
Chromatin dependent repression mechanisms. (A) Total histone H1 levels in LTH and NLT cells were measured by ChIP. No significant difference was found in total histone H1 on the MMTV promoter. (B) Phospho-histone H1 levels measured by ChIP on the MMTV promoter were not significantly different in uninduced NLT and LTH cells. No hormone-dependent decline in phospho-histone H1 level was found in response to hormone induction on the repressed MMTV promoter in LTH cells. (C) Brg1-dependent RNA expression was measured by RT- PCR of CD44 and CSF1. No significant loss of expression was found in LTH compared with NLT cells.