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. Author manuscript; available in PMC: 2009 Mar 17.
Published in final edited form as: J Neurochem. 2007 Jan 24;101(2):555–565. doi: 10.1111/j.1471-4159.2006.04394.x

Figure 1.

Figure 1

In vitro model of the BBB. Primary BMEC were cultured for 2 days in the presence of puromycin to yield a nearly 100% pure BMEC monolayer on the collagen IV/fibronectin-coated filter support. On DIV 2 BMEC were switched to puromycin-free medium. After reaching confluence on DIV 3, NPC were added to the lower compartment in the presence or absence of mitogens (FGF-2, EGF). The resulting co-cultures were used to assess NPC effects on the BBB properties of the BMEC monolayer.