Skip to main content
. Author manuscript; available in PMC: 2009 Mar 17.
Published in final edited form as: J Neurochem. 2007 Jan 24;101(2):555–565. doi: 10.1111/j.1471-4159.2006.04394.x

Figure 2.

Figure 2

Influence of NPC co-culture and NPC-conditioned media on BMEC TEER. BMEC were cultured as follows: 1. in the absence of NPC (control), 2. in the presence of differentiating NPC (+NPC −mitogens), 3. in the presence of 24h pre-differentiated NPC (+NPC 24h prediff), 4. in NPC-conditioned medium generated by 24 hour of NPC culture in mitogen-free differentiation medium (NPC conditioned −mitogens), 5. in the presence of NPC in mitogen-containing medium (NPC +mitogens), and 6. in 24 hour conditioned mitogen-containing medium from dividing NPC (NPC conditioned +mitogens). The TEER was monitored after 24 hours for each culture condition and expressed as normalized % of control TEER values. The mitogen-containing conditions (5 and 6) were independently normalized to a mitogen-containing BMEC monoculture control. The statistical significance is given with p < 0.001 (**) and p < 0.03 (*) as determined by the unpaired Student’s t-test. Triplicate cultures were analyzed for each condition and results display mean ±SD. The results are representative of 8 independent experiments where the increase in TEER in the presence of differentiating NPC (2) ranged from 17–53%.