FIG. 2.

Enhanced short-term facilitation in the dfmr1;dmGluRA double mutant. A: representative EJC traces during a 1-s, 20-Hz stimulation train in the genetic background control (top), dmfr1 single mutant null (2nd), dmGluRA single mutant null (3rd), and dmfr1;dmGluRA double null mutant (bottom). The dfmr1;dmGluRA double null shows strongly enhanced short-term facilitation compared with control, dfmr1, and dmGluRA. Scale bars: 5 nA, 15 ms. B: quantification of mean normalized EJC amplitudes for the 1st 20 responses of stimuli trains for each genotype. All responses were normalized to the first EJC amplitude of the 20-Hz stimulus train. Each animal was tested 5 times, and the mean of the normalized responses was averaged with all animals from that genotype. Facilitation is significantly enhanced in dfmr1;dmGluRA, with no significant difference between control, dfmr1, and dmGluRA. Sample sizes: control (n = 6), dfmr1 (n = 7), dmGluRA (n = 7), and dfmr1;dmGluRA (n = 7).