TABLE 1.
Ionic concentrations of pipette solutions (in mM)
| Solution | Cl− | Na+ | K+ | Gluconate− | Osmolarity, mOsm | Junction Potential, mV |
|---|---|---|---|---|---|---|
| “17” | 17.2 | 5.1 | 130 | 135 | 299 | −17 |
| “32” | 32 | 5.1 | 130 | 120 | 298 | −12 |
| “52” | 52.2 | 5.1 | 130 | 100 | 281 | −10 |
| “77” | 77.2 | 5.1 | 130 | 75 | 278 | −9 |
| “157” | 157.2 | 10.0 | 130 | 0 | 286 | −5 |
All solutions also included (in mM): MgCl2 (1), CaCl2 (0.1), HEPES (10), ATP-Na (1), GTP-Mg (0.1), BAPTA (10); and voltage-gated channel blockers (in mM): verapamil (0.1), QX-314 (10), TEA-Cl (10), and CsCl (5). Junction potential was adjusted on-line.