Table 1.
Macaque liver tissues used for transcriptome analyses
| Liver samples | AST/ALTd | Glucosee | Triglyceridesf | Virus titerg |
| Uninfected controls | ||||
| Rh Ctrl7 | 38/65 | 74 | 65 | <102 |
| Rh Ctrl8 | 24/53 | 79 | 61 | <102 |
| Rh Ctrl9 | 47/71 | 87 | 77 | <102 |
| LCMV-WE1-Pre-viremic | ||||
| Rh 1WE (day 1)a | 44/61 | 53 | 27 | <102 |
| Rh 2WE (day 1) | 63/54 | 58 | 30 | <102 |
| Rh 3WE (day 2) | 59/37 | 60 | 18 | <102 |
| Rh 5WE (day 3) | 27/71 | 62 | 37 | <102 |
| LCMV-WE2-Viremicb | ||||
| Rh 6WE (day 4) | 96/137 | 71 | 33 | <102 |
| Rh 7WE (day 4) | 24/38 | 67 | 18 | <102 |
| Rh 8WE (day 6) | 26/56 | 54 | 32 | 1.4 × 104 |
| Rh 9WE (day 6) | 125/82 | 70 | 31 | 5.0 × 103 |
| Rh 10WE (day 7) | 190/325 | 66 | 30 | 1.5 × 106 |
| LCMV-WE3 Terminal | ||||
| Rh 11WE (day 11) | 642/587 | 30 | 235 | 5.5 × 105 |
| Rh 12WE (day 12) | 1504/681 | 40 | 1402 | 1.0 × 106 |
| LCMV-not diseasedc | ||||
| Rh 3ARM (day 3) | 45/74 | 70 | 75 | <102 |
| Rh 5ARM (day 5) | 18/40 | 78 | 62 | <102 |
| Rh 1ARM/WE-1 | 52/24 | 65 | 79 | <102 |
| Rh 1ARM/WE-5 | Not done | Not done | ND | <102 |
| Rh 2ARM/WE-6 | 56/34 | 63 | 80 | <102 |
| Rh 2ARM/WE-2 | Not done | Not done | ND | <102 |
a (day 1) is the day of necropsy after infection. Each sample is from the liver of a different rhesus macaque.
b Pre-viremic means liver was harvested day 1, day 2 or day 3 after infection; Viremic means liver was harvested day 4 to day 7, and Terminal means liver was harvested day 11 or 12 during the terminal stage of disease. ND = Not done.
c Animals that were LCMV-infected but not diseased were either infected with LCMV-ARM and sacrificed day 3 or day 5 after infection, or they were infected with LCMV-ARM and LCMV-WE (103 pfu each virus) and sacrificed on day 30 after infection.
d AST/ALT means the ratio of plasma levels of aspartate aminotransferase to alanyl aminotransferase in international units per liter (IU/L). Normal ranges are AST/ALT = 18–82/22–87 IU/L. Values outside the reference range are bolded.
e Serum glucose levels are in mg/dL with a reference range of 55–80 mg/dL (Animal Diagnostics Laboratory, Baltimore, MD.)
f Serum triglyceride levels have a reference range of 50–82 mg/dL. Other parameters derived from blood analysis include red blood cells, hemoglobin levels and cholesterol levels which were all within normal ranges for all samples.
g Virus was titered on Vero cell monolayers as described [2], and given as plaque forming units per gram of liver tissue. Samples in the viremic group that had undetectable titers (<102 pfu/gm) were positive for virus nucleic acid by RT-PCR and virus co-cultivation as described previously [3].