Table 3.
Expression of genes involved with TCA cycle and glucose metabolism in LCMV-WE infected macaque liver
| Pre-viremic | Viremic | |||||
| GenBank Accession no. | Symbol | Gene description | Fold changea | pb | Fold changea | pb |
| Citric acid cycle | ||||||
| NM_001096 | ACLY | ATP citrate lyase | 2.14 | 0.0809 | 3.58 | 0.0040 |
| AI363836 | FH | Fumarate hydratase | 5.54 | 0.0045 | 3.05 | 0.0356 |
| AI826060 | IDH3A | Isocitrate dehydrogenase 3 (NAD+) alpha | 1.51 | 0.4150 | 2.29 | 0.0440 |
| AF023266 | IDH3B | Isocitrate dehydrogenase 3 (NAD+) beta | 2.41 | 0.0219 | 2.19 | 0.0290 |
| NM_004135 | IDH3G | Isocitrate dehydrogenase 3 (NAD+) gamma | 2.14 | 0.0262 | 2.29 | 0.0122 |
| NM_005917 | MDH1 | Malate dehydrogenase 1, NAD (soluble) | 9.51 | 0.0056 | 9.25 | 0.0039 |
| BC001917 | MDH2 | Malate dehydrogenase 2, NAD (mitochondrial) | 2.77 | 0.0739 | 3.48 | 0.0221 |
| NM_004168 | SDHA | Succinate dehydrogenase complex, A, flavoprotein | 4.17 | 0.0108 | 4.03 | 0.0087 |
| NM_003000 | SDHB | Succinate dehydrogenase complex, B, iron sulfur | 5.98 | 0.0120 | 6.68 | 0.0056 |
| AF080579 | SDHC | Succinate dehydrogenase complex, C, membrane protein | 14.12 | 0.0003 | 11.63 | 0.0003 |
| AL050226 | SUCLG2 | Succinate-CoA ligase, GDP-forming, beta | 4.23 | 0.0108 | 2.83 | 0.0442 |
| Glycolysis | ||||||
| NM_000034 | ALDOA | Aldolase A, fructose-bisphosphate | 2.03 | 0.0136 | 3.05 | 0.0004 |
| AK026411 | ALDOB | Aldolase B, fructose-bisphosphate | 14.83 | 0.0002 | 6.02 | 0.0027 |
| AK026525 | GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | 6.59 | 0.0034 | 4.89 | 0.0066 |
| M69051 | GCK | Glucokinase (hexokinase 4) | 3.92 | 0.0010 | 1.01 | 0.9980 |
| S81916 | PGK1 | Phosphoglycerate kinase 1 | 23.59 | 0.0009 | 23.43 | 0.0005 |
| NM_002633 | PGM1 | Phosphoglucomutase 1 | 6.28 | 0.0038 | 5.03 | 0.0058 |
| Glycogen metabolism | ||||||
| S70004 | GYS2 | Glycogen synthase 2, liver | 9.65 | 0.0004 | 5.54 | 0.0020 |
| NM_002863 | PYGL | Phosphorylase glycogen, liver | 4.96 | 0.0120 | 3.14 | 0.0485 |
| NM_002633 | PGM1 | Phosphoglucomutase 1 | 6.28 | 0.0038 | 5.03 | 0.0058 |
| Gluconeogenesis | ||||||
| D26054 | FBP1 | Fructose-1,6-bisphosphatase 1 | 4.08 | 0.0061 | 1.59 | 0.1559 |
| BC020700 | G6PC | Glucose-6-phosphatase, catalytic | 12.13 | 0.0034 | 5.17 | 0.0265 |
| Others | ||||||
| NM_000284 | PDHA1 | Pyruvate dehydrogenase (lipoamide) alpha 1 | 3.48 | 0.0769 | 4.53 | 0.0249 |
a Mean fold changes were calculated using a division of raw expression values between experimental sample and uninfected control.
b All samples were analyzed separately. Changes in gene expression with a cutoff of 2.0-fold increased or decreased expression was used and, the p-value was calculated by Student's t-test. Data are displayed only where the, p ≤ 0.05. This p-value was used as a measure of the magnitude of the difference between groups and to determine significance of the modulation. The modulated genes in the pathway are listed in alphabetic order.