Table 4.
Expression of genes involved with fatty acid oxidation in LCMV-WE infected macaque liver
| Pre-viremic | Viremic | |||||
| GenBank Accession no. | Symbol | Gene description | Fold changea | pb | Fold changea | pb |
| Mitochondrial {beta}-oxidation | ||||||
| BE897866 | ACADSB | Acyl-CoA dehydrogenase, short/branched chain | 1.77 | 0.6240 | -2.93 | 0.0190 |
| NM_004453 | ETFDH | Electron-transferring flavoprotein dehydrogenase | 3.10 | 0.0019 | 1.49 | 0.1412 |
| NM_004092 | ECHS1 | Enoyl CoA hydratase, short chain, 1, mitochondrial | 7.06 | 0.0110 | 4.79 | 0.0260 |
| U04627 | HADHA | Hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha | 3.43 | 0.0180 | 3.75 | 0.0086 |
| Microsomal oxidation | ||||||
| AF182275 | CYP2A6 | Cytochrome P450, family 2, subfamily A, polypeptide 6 | 17.88 | 0.0008 | 7.21 | 0.0009 |
| X06399 | CYP2B6 | Cytochrome P450, family 2, subfamily B, polypeptide 6 | 3.16 | 0.0079 | -1.02 | 0.9980 |
| NM_030878 | CYP2C8 | Cytochrome P450, family 2, subfamily C, polypeptide 8 | 3.51 | 0.0020 | 2.35 | 0.0147 |
| NM_000106 | CYP2D6 | Cytochrome P450, family 2, subfamily D, polypeptide 6 | 7.78 | 0.0065 | 2.66 | 0.1530 |
| AF182276 | CYP2E1 | Cytochrome P450, family 2, subfamily E, polypeptide 1 | 4.06 | 0.0150 | 2.87 | 0.0459 |
| NM_000777 | CYP3A5 | Cytochrome P450, family 3, subfamily A, polypeptide 5 | 7.57 | 0.0005 | 6.36 | 0.0005 |
| AF315325 | CYP3A7 | Cytochrome P450, family 3, subfamily A, polypeptide 7 | 11.39 | 0.0006 | 5.28 | 0.0050 |
| Ketogenesis | ||||||
| BC000408 | ACAT2 | Acetyl-CoA acetyltransferase 2 (acetoacetyl CoA thiolase) | 6.10 | 0.0065 | 4.78 | 0.0107 |
| BG035985 | HMGCS1 | 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) | 3.10 | 0.0198 | 4.69 | 0.0467 |
| NM_005518 | HMGCS2 | 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial) | 5.16 | 0.0135 | 2.91 | 0.0748 |
| BF224073 | TCP1 | T-complex 1 | 3.43 | 0.0332 | 2.50 | 0.0977 |
| Peroxisomal oxidation | ||||||
| NM_003500 | ACOX2 | Acyl-CoA oxidase 2, branched chain | 6.63 | 0.0019 | 3.12 | 0.0267 |
| NM_004092 | ECHS1 | Enoyl CoA hydratase, short chain, 1, mitochondrial | 7.06 | 0.0110 | 4.79 | 0.0260 |
| U04627 | HADHA | Hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha | 3.43 | 0.0180 | 3.75 | 0.0086 |
| NM_005525 | HSD11B1 | Hydroxysteroid (11-beta) dehydrogenase 1 | 2.80 | 0.0325 | 2.20 | 0.0874 |
| AL031228 | HSD17B8 | Hydroxysteroid (17-beta) dehydrogenase 8 | 2.80 | 0.0258 | 2.20 | 0.0620 |
| Other peroxisomal proteins | ||||||
| AU147084 | CAT | Catalase | 8.63 | 0.0028 | 5.78 | 0.0069 |
| Others | ||||||
| NM_000072 | CD36 | CD36 molecule (thrombospondin receptor) | 48.18 | 0.0001 | 16.11 | 0.0008 |
a Mean fold changes were calculated using a division of raw expression values between experimental sample and uninfected control.
b All samples were analyzed separately. Changes in gene expression with a cutoff of 2.0-fold increased or decreased expression was used and, the p-value was calculated by Student's t-test. Data are displayed only where the, p ≤ 0.05. This p-value was used as a measure of the magnitude of the difference between groups and to determine significance of the modulation. The modulated genes in the pathway are listed in alphabetic order.