Table 7.
Expression of genes involved with complement and coagulation cascades in LCMV-WE-infected macaque liver
| Pre-viremic | Viremic | |||||
| GenBank Accession no. | Symbol | Gene description | Fold changea | pb | Fold changea | pb |
| NM_000014 | A2M | Alpha-2-macroglobulin | 3.31 | 0.0219 | 2.71 | 0.0417 |
| NM_000491 | C1QB | Complement component 1, q subcomponent, B chain | 3.65 | 0.1140 | 12.2 | 0.0029 |
| AI184968 | C1QC | Complement component 1, q subcomponent, C chain | 1.47 | 0.5402 | 3.27 | 0.0140 |
| AL573058 | C1R | Complement component 1, r subcomponent | 2.20 | 0.0287 | 2.73 | 0.0054 |
| BC007010 | C1S | Complement component 1, s subcomponent | 53.44 | 0.0006 | 50.21 | 0.0004 |
| NM_000592 | C4A/C4B | Complement component 4A (Rodgers blood group)///complement component 4B (Childo blood group) | 5.20 | 0.0002 | 6.32 | 0.0001 |
| NM_000716 | C4BPB | Complement component 4 binding protein, beta | 3.22 | 0.0401 | 5.65 | 0.0032 |
| NM_001735 | C5 | Complement component 5 | 8.75 | 0.0015 | 7.83 | 0.0013 |
| J05064 | C6 | Complement component 6 | 3.24 | 0.0332 | 2.63 | 0.0624 |
| NM_000587 | C7 | Complement component 7 | 2.34 | 0.1850 | 4.28 | 0.0152 |
| M17263 | C8G | Complement component 8, gamma polypeptide | 4.72 | 0.0051 | 4.56 | 0.0038 |
| AL570661 | CD46 | CD46 molecule, complement regulatory protein | 4.72 | 0.0015 | 3.24 | 0.0060 |
| X04697 | CFH | Complement factor H | 2.90 | 0.0124 | 2.86 | 0.0091 |
| NM_000506 | F2 | Coagulation factor II (thrombin) | 2.11 | 0.0329 | 1.65 | 0.1360 |
| AA910306 | F5 | Coagulation factor V (proaccelerin, labile factor) | 3.16 | 0.0218 | 2.47 | 0.0071 |
| NM_000504 | F10 | Coagulation factor X | 2.42 | 0.0070 | 1.85 | 0.0513 |
| NM_000128 | F11 | Coagulation factor XI (plasma thromboplastin antecedent) | -2.28 | 0.0150 | -2.75 | 0.0029 |
| NM_021871 | FGA | Fibrinogen alpha chain | 3.86 | 0.0035 | 3.48 | 0.0039 |
| BG545288 | FGB | Fibrinogen beta chain | 49.86 | 0.0001 | 45.56 | 0.0001 |
| NM_000509 | FGG | Fibrinogen gamma chain | 2.67 | 0.0355 | 2.56 | 0.0325 |
| M74220 | LPA/PLG | Lipoprotein, Lp(a)///plasminogen | 4.69 | 0.0256 | 3.81 | 0.0396 |
| NM_000892 | KLKB1 | Kallikrein B, plasma (Fletcher factor) 1 | -1.70 | 0.1730 | -2.32 | 0.0207 |
| AI274095 | MASP1 | Mannan-binding lectin serine peptidase 1 (C4/C2 activating component of Ra-reactive factor) | 3.07 | 0.0004 | 2.78 | 0.0005 |
| AB008047 | MASP2 | Mannan-binding lectin serine peptidase 2 | 4.75 | 0.0144 | 3.16 | 0.0489 |
| NM_000242 | MBL2 | Mannose-binding lectin (protein C) 2, soluble (opsonic defect) | 3.18 | 0.0162 | 1.37 | 0.5910 |
| BE880828 | MCFD2 | Multiple coagulation factor deficiency 2 | 11.71 | 0.0004 | 14.72 | 0.0001 |
| M74220 | PLG | Plasminogen | 3.86 | 0.0059 | 3.60 | 0.0054 |
| NM_000295 | SERPINA1 | Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), 1 | 8.20 | 0.0003 | 7.21 | 0.0004 |
| BC022309 | SERPINC1 | Serpin peptidase inhibitor, clade C (antithrombin), member 1 | 30.65 | 0.0005 | 19.29 | 0.0009 |
| NM_000062 | SERPING1 | Serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 | 2.77 | 0.0336 | 3.07 | 0.0296 |
| BF511231 | TFPI | Tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) | 2.05 | 0.0312 | 1.45 | 0.2590 |
a Mean fold changes were calculated using a division of raw expression values between experimental sample and uninfected control.
b All samples were analyzed separately. Changes in gene expression with a cutoff of 2.0-fold increased or decreased expression was used and, the p-value was calculated by Student's t-test. Data are displayed only where the, p ≤ 0.05. This p-value was used as a measure of the magnitude of the difference between groups and to determine significance of the modulation. The modulated genes in the pathway are listed in alphabetic order.