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. 2009 Feb 9;10:72. doi: 10.1186/1471-2164-10-72

Table 4.

Results of EST assembly for each Mediterranean mussel cDNA library.

Library Total ESTs Discared ESTs Analyzed ESTs # EST in cluster # clusters # singletons # consensus % poly(A) detection % gene discovery % redundancy
DiG01 93 24 69 54 31 15 46 66.7 21.7 78.3
DiG02 95 23 72 57 28 15 43 73.6 23.6 76.4
Ese00 285 121 164 143 90 21 111 34.8 12.8 87.2
GDG01 35 10 25 19 19 6 25 64.0 24.0 76.0
Gll01 95 18 77 45 37 32 69 51.9 41.6 58.4
Hae01 656 93 563 511 237 52 289 56.0 10.3 89.7
Hae02 540 100 440 352 196 88 284 41.4 21.4 78.6
Hae03 523 130 393 354 126 39 165 83.2 10.9 89.1
Hae04 568 116 452 411 130 41 171 81.2 9.5 90.5
Hae05 475 134 341 309 110 32 142 83.3 10.0 90.0
MxT01 767 200 567 381 260 186 446 34.0 41.1 58.9
MxT02 768 577 191 138 90 53 143 39.8 37.2 62.8
MxT03 4224 1478 2746 2038 586 708 1294 50.6 38.2 61.8
MxT04 74 22 52 52 50 0 50 61.5 0.0 100.0
Nor01 15621 3034 12587 9228 2137 3359 5496 23.3 65.6 34.4
Tst00 96 55 41 25 24 16 40 53.7 39.0 61.0
Tst01 24 16 8 5 5 3 8 25.0 37.5 62.5
Overall 24939 6151 18788 14122 4156 4666 8822 54.3 26.1 73.9

Total ESTs = number of produced chromatograms; Discarded ESTs = number of low quality ESTs; Analyzed ESTs = number of sequences processed for clustering; # EST in cluster = number of sequences in cluster; # clusters = total number of clusters; # singletons = number of putative transcripts identified by one single EST; # consensus = number of non-redundant sequences; % poly(A) detection = percentage of sequences where poly(A) has been identified and trimmed; % gene discovery = percentage of ESTs identifying putative new transcripts in the total EST analyzed from each library; % redundancy = percentage of sequences also identified by other cDNA libraries.

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