Table 4.
Results of EST assembly for each Mediterranean mussel cDNA library.
Library | Total ESTs | Discared ESTs | Analyzed ESTs | # EST in cluster | # clusters | # singletons | # consensus | % poly(A) detection | % gene discovery | % redundancy |
DiG01 | 93 | 24 | 69 | 54 | 31 | 15 | 46 | 66.7 | 21.7 | 78.3 |
DiG02 | 95 | 23 | 72 | 57 | 28 | 15 | 43 | 73.6 | 23.6 | 76.4 |
Ese00 | 285 | 121 | 164 | 143 | 90 | 21 | 111 | 34.8 | 12.8 | 87.2 |
GDG01 | 35 | 10 | 25 | 19 | 19 | 6 | 25 | 64.0 | 24.0 | 76.0 |
Gll01 | 95 | 18 | 77 | 45 | 37 | 32 | 69 | 51.9 | 41.6 | 58.4 |
Hae01 | 656 | 93 | 563 | 511 | 237 | 52 | 289 | 56.0 | 10.3 | 89.7 |
Hae02 | 540 | 100 | 440 | 352 | 196 | 88 | 284 | 41.4 | 21.4 | 78.6 |
Hae03 | 523 | 130 | 393 | 354 | 126 | 39 | 165 | 83.2 | 10.9 | 89.1 |
Hae04 | 568 | 116 | 452 | 411 | 130 | 41 | 171 | 81.2 | 9.5 | 90.5 |
Hae05 | 475 | 134 | 341 | 309 | 110 | 32 | 142 | 83.3 | 10.0 | 90.0 |
MxT01 | 767 | 200 | 567 | 381 | 260 | 186 | 446 | 34.0 | 41.1 | 58.9 |
MxT02 | 768 | 577 | 191 | 138 | 90 | 53 | 143 | 39.8 | 37.2 | 62.8 |
MxT03 | 4224 | 1478 | 2746 | 2038 | 586 | 708 | 1294 | 50.6 | 38.2 | 61.8 |
MxT04 | 74 | 22 | 52 | 52 | 50 | 0 | 50 | 61.5 | 0.0 | 100.0 |
Nor01 | 15621 | 3034 | 12587 | 9228 | 2137 | 3359 | 5496 | 23.3 | 65.6 | 34.4 |
Tst00 | 96 | 55 | 41 | 25 | 24 | 16 | 40 | 53.7 | 39.0 | 61.0 |
Tst01 | 24 | 16 | 8 | 5 | 5 | 3 | 8 | 25.0 | 37.5 | 62.5 |
Overall | 24939 | 6151 | 18788 | 14122 | 4156 | 4666 | 8822 | 54.3 | 26.1 | 73.9 |
Total ESTs = number of produced chromatograms; Discarded ESTs = number of low quality ESTs; Analyzed ESTs = number of sequences processed for clustering; # EST in cluster = number of sequences in cluster; # clusters = total number of clusters; # singletons = number of putative transcripts identified by one single EST; # consensus = number of non-redundant sequences; % poly(A) detection = percentage of sequences where poly(A) has been identified and trimmed; % gene discovery = percentage of ESTs identifying putative new transcripts in the total EST analyzed from each library; % redundancy = percentage of sequences also identified by other cDNA libraries.