Figure 1. Intercellular and Intracellular calcium waves in sustentacular cells.

(A) Shown are a series of images acquired during a spontaneous intercellular calcium wave in a Fluo-4AM loaded slice. See supplemental data for movie. Scale bar, 20 μm. (B) Example of a spontaneous intracellular calcium wave traveling from the apical to the basal cell layer. Numbered regions correspond to the traces shown in (C). Dashed line, basement membrane. Scale bar, 20 μm. (C) Time course of the transient Ca²⁺ increase recorded from the sustentacular cell shown in B. Numbered traces correspond to the regions shown in (B). (D–F) Confocal image (D) and time course of the UTP evoked Ca²⁺ transients (E) and inherent fluorescein (F) fluorescence recorded from the sustentacular cell shown in (D). Numbered traces correspond to the regions shown in (D). ▲, time of loop injection of UTP or fluorescein.