Figure 6. The PLC pathway has a role in purinergic-evoked calcium increases.
(A–C, E) Slices were superfused with multiple applications of 10 μM UTP in the presence of (A) 150 μM neomycin, (B) 100 μM U73122, (C) 100 μM U73343, (E) 100 μM 2-APB. (▲indicates time of UTP superfusion, □ indicates time of drug superfusion). Y axis is normalized peak amplitude. Right panels: Average normalized peak amplitudes and the predicted peak amplitudes for the second application (v) are shown. *, p<0.01. (D) UTP responsive cells (mean ± s.e.m.) from slices incubated 20 min in 0.1% DMSO vehicle (n=4) or 200 μM U73122 (n=10, p=0.0008).