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. 2009 Apr 3;5(4):e1000362. doi: 10.1371/journal.ppat.1000362

Figure 2. Opioid peptide release from neutrophils is triggered by mycobacteria and FPR agonists but not by TLR-2 or TLR-4 agonists.

Figure 2

[A–C] Rat and human neutrophils as well as CD14+ human monocytes were incubated with heat-inactivated Mycobacterium butyricum (Myco. but.), and Met-enkephalin (ENK) release was quantified by radioimmunoassay (n = 7–13 * p<0.05, one way RM ANOVA, Student-Newman-Keuls Method). [D, E] Expression of TLR-2, TLR-4 [D] and FPR [E] was determined on human neutrophils by flow cytometry (dotted line: unstained control, grey histogram: isotype control, black line: anti-TLR-2-PE, black histogram: fMLP-FITC or anti-TLR-4-PE). [F–H] Human (n = 7–16) and [I–K] rat neutrophils (n = 7–21) were stimulated with the TLR-2 agonist peptidoglycan, the TLR-4 agonist lipopolysaccharide or the FPR agonist fMLP, and Met-enkephalin (ENK) release was measured in the supernatant (* p<0.05; one way RM ANOVA, Student-Newman-Keuls Method). Data are presented as means±SEM.