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Mock [A] or human FPR [B,C] transfected HEK293 cells or human neutrophils [D–F] were loaded with Fura-2. Changes in [Ca2+]i were analyzed after addition of Mycobacterium butyricum (Myco. but.) at 60 s in the presence [C, E] or absence [B,D] of 10 µM Boc-FLFLF (Boc). [F] Impact of fMLP stimulation on subsequent stimulation with Mycobacterium butyricum in human neutrophils.
