Figure 2. MTMR4 and Nedd4 co-localize to late endosomes/recycling endosomes in living cells.
(A) Immunofluorescence of HeLa cells transfected with HA-tagged hMTMR4 (red) and GFP–hNedd4 (green). Overlay shows merging of the two images. (B) Co-localization of HA–hMTMR4 with markers of late endosomes (Rab7) and recycling endosomes (transferrin receptor). HA–hMTMR4 does not co-localize with markers of early endosomes (Rab5). (C) Triple labelling of HeLa cells transfected with HA–hMTMR4 (blue), GFP–Nedd4 (green) and PX–mCherry (red) to represent PtdIns3P localization. Arrowheads point to examples of co-localization. Mander's co-localization coefficients, r (degrees of freedom), are shown for each set of co-staining. Scale bars, 11 μm.