Figure 4. MTMR4 and Nedd4 are differentially regulated in atrophied muscle.

(A) Representative Western blot of MTMR4, Nedd4 and HPRT (as loading control) from gastrocnemius muscle insoluble protein fractions from denervated (Den) and contralateral control (Con) limbs. MTM tx is positive control lysate from MTMR4 transfected or untransfected (untx) HEK-293 cells. Molecular masses are indicated in kDa. (B) Quantification of the chemiluminescent signal from Western blots performed using Bio-Rad Fluor-S Max acquisition system and Quantity One software. Each bar represents the results from eight muscles (mean+S.E.M.). P<0.05 denotes statistically significant differences between mean values. CNT, counts.