Fig. 4.

A and B: Gene-expression analysis of differentiation of traumatized muscle-derived multiprogenitor cells. A: Traumatized muscle-derived multiprogenitor cells were cultured in growth medium (GM), osteogenic induction medium (OM), or adipogenic induction medium (AM) and then were lysed and RNA-extracted. Gene-expression profiles were then analyzed with use of reverse transcription polymerase chain reaction. Cells cultured in osteogenic induction medium showed upregulated expression of CBFA1/RUNX2, alkaline phosphatase (ALP), and osteocalcin, characteristic of osteoblastic phenotype. Cells cultured in adipogenic induction medium exhibited upregulated expression of PPARγ2, lipoprotein lipase (LPL), and fatty acid binding protein 4 (FABP4), characteristic of adipocytic phenotype. B: Pellet cultures of multiprogenitor cells in chondrogenic medium expressed the chondrogenic genes SOX9, COL2A1, and aggrecan (AGC). The expression of COL2A1 was upregulated from Day 7 to Day 21, suggesting chondrocyte maturation. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression was analyzed as a control for RNA loading.