TABLE I.
Gene* | Primer Sequence | Annealing Temperature | No. of Cycles Performed | Product Size (no. of base pairs) |
---|---|---|---|---|
GAPDH | ||||
Forward | CAAGGCTGAGAACGGGAAGC | 58°C | 26 | 194 |
Reverse | AGGGGGCAGAGATGATGACC | |||
COL1A2 | ||||
Forward | GGCTCCTGCTCCTCTTAGCG | 58°C | 26 | 132 |
Reverse | CATGGTACCTGAGGCCGTTC | |||
CBFA1/RUNX2 | ||||
Forward | ACTGGGCCCTTTTTCAGA | 53°C | 26 | 317 |
Reverse | GCGGAAGCATTCTGGAA | |||
ALP | ||||
Forward | TCAGAAGCTCAACACCAACG | 55°C | 26 | 199 |
Reverse | GTCAGGGACCTGGGCATT | |||
Osteocalcin | ||||
Forward | GCCTTTGTGTCCAAGC | 52°C | 28 | 315 |
Reverse | GGACCCCACATCCATAG | |||
PPARγ2 | ||||
Forward | TGAATGTGAAGCCCATTGAA | 53°C | 26 | 161 |
Reverse | CTGCAGTAGCTGCACGTGTT | |||
LPL | ||||
Forward | GAGATTTCTCTGTATGGCACC | 52°C | 26 | 276 |
Reverse | CTGCAAATGAGACACTTTCTC | |||
FABP4 | ||||
Forward | TGGGCCAGGAATTTGACGAAGT | 59°C | 24 | 215 |
Reverse | TCAACGTCCCTTGGCTTATGCT | |||
SOX9 | ||||
Forward | ACATCTCCCCCAACGCCATC | 58°C | 34 | 173 |
Reverse | TCGCTTCAGGTCAGCCTTGC | |||
COL2A1 | ||||
Forward | GGAAACTTTGCTGCCCAGATG | 58°C | 34 | 167 |
Reverse | TCACCAGGTTCACCAGGATTGC | |||
Aggrecan | ||||
Forward | TGCGGGTCAACAGTGCCTATC | 58°C | 36 | 182 |
Reverse | CACGATGCCTTTCACCACGAC |
GAPDH = Glyceraldehyde 3-phosphate dehydrogenase. ALP = Alkaline phosphatase. PPARγ2 = peroxisome proliferator-activated receptors-γ2. LPL = Lipoprotein lipase. FABP4 = fatty acid binding protein 4.