Table 1. Activity of sphingosine and ceramide kinases in the presence of lipids.
Values are percentage activity, relative to vehicle control, for sphingosine or ceramide kinases in the presence of 10 μM test lipid. Vehicle was methanol for all compounds except cerebroside (DMSO vehicle).
| Lipid | Sphk2 | Sphk1 | Cerk |
|---|---|---|---|
| galactosylceramide 3-sulfate (sulfatide) | 37 | 156 | 36 |
| galactosylceramide (cerebroside) | 84 | 109 | 101 |
| C16 sulfatide | 47 | ND | 22 |
| C2 sulfatide | 160 | ND | 99 |
| cholesterol | 107 | ND | 109 |
| phosphatidylserine | 203 | ND | 65 |
| phosphatidic acid | 102 | 265 | 102 |
| phosphatidylinositol | 187 | ND | 62 |
| phosphatidylinositol 4-phosphate | 89 | 175 | 102 |
| phosphatidylinositol 4,5-bisphosphate | 60 | 99 | 94 |
ND: not determined. Results are the mean of triplicate assays.