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(A) Total protein from wild type and β-catenin knock out mice were analyzed by western blot with the Y654-β-catenin antibody. (B) Total protein from primary rat hepatocytes treated with FBS, HGF, EGF or no serum was analyzed by western blot for Y654-β-catenin and total β-catenin. Ponceau Red staining verified equal loading of protein in both instances.
