Fig. 5.

RNA synthesis activity of PsV-F virions. RNA synthesis was performed by using purified PsV-F virions in a standard reaction buffer including [α-³²P]GTP. Reaction products were treated with various amounts of RNase V1 (lanes 3–5 and 10–12, in units) or RNase A (lanes 6–8 and 13–15, in ng) for 20 min at room temperature, before (lanes 2–8) or after (lanes 9–15) the total RNA was extracted by using phenol:chloroform. The control samples (lanes 2 and 9) had no RNase treatment. All RNA samples were reextracted and subjected to agarose gel electrophoresis, and the resulting gel was stained with ethidium bromide (EB; Upper) and photographed before drying for autoradiography (³²P; Lower).