Fig. 5.

Depolarization and histone acetylation increase RNA pol II processivity. (A) Scheme showing the proximal and distal amplicons used for qPCR assessment. (B) Increase in D/P pre-mRNA accumulation. Cells were treated for 4 to 6 h with high KCl (Depol) or TSA (3.3 μM). Bars correspond to the normalized means and SEs for the pooled data of 3 (Control and Depol) or 2 different experiments (TSA). The table below shows the results of a 2-way ANOVA used to analyze statistical significance, considering the different treatments as one factor and the different experiments as the other factor (no significant effect of experiment or interaction between the two was found). (C) Depolarization after recovery in normal medium. Cells were treated for 4 h and recovered in normal medium for 20 more hours. Bars correspond to the normalized means and SEs of one experiment in triplicate.