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Melting curves of forked DNA constructs. (A) UV melting curves at 260 and 320 nm for forked constructs containing site-specific pairs of 2-AP probes. □, 260 nm, {1,2}; ○, 260 nm, {−2,−1}; solid line, 320 nm, {1,2}; dashed line, 320 nm, {−2,−1}. (B) CD melting curves at 330 nm for forked constructs containing 2-AP dimer probes. ○, {−1,1}; ■, {1,2}; ●, {3,4}; □, {4,5}.
