Fig. 3.
The effect of ABA on BR signaling outputs does not depend on the BR receptor complex. (A) The effect of ABA on the expression of BR-responsive genes in bri1-116; bri1-116 is a null allele of bri1 and contains a stop codon in the extracellular domain. Were treated 20-day old seedlings with 1 μM ABA. (B) The subcellular localization of BKI1-YFP after ABA treatment. Were treated 4-day old seedlings of BKI1-YFP-OX with 1 μM ABA for 15 min or 30 min. (C) ABA does not alter the subcellular localization of BKI1-YFP after BL treatment. Were pretreated 4-day old Seedlings of BKI1-YFP-OX with 1 μM BL for 10 min; then, they were treated with 1 μM ABA for 15 min or 30 min. (D) ABA does not alter the subcellular localization of BKI1-YFP in bri1-116 background. Were treated 4-day old seedlings of BKI1-YFP-OX/bri1-116 with 1 μM ABA for 15 or 30 min.