Fig. 5.

The role of ABA on BR signaling is through ABI2 and ABI1. (A) The slight effect of ABA on the expression of BR-responsive genes in ABA signaling mutants. Nine-day old seedlings of ABA signaling mutants (abi1 and abi2) were treated with 1 μM ABA. (B) The alteration of the effect of ABA on phosphorylation status of BES1 in ABA signaling mutants. Nine-day old seedlings of ABA signaling mutants abi1 and abi2 were pretreated with 1 μM BL for 2 h; then, with 1 μM ABA for 18 h. Seedlings pretreated with BL for 2 h as “−,” and pretreated with BL for 2 h then pretreated with ABA for 18 h as “+.” Immunoblot with anti-BES1 shows the levels of p-BES1 and BES. Ponceau-S stained Rubisco large subunit serves as a loading control.