Fig. 1.

Levels of the peroxisomal enzymes isocitrate lyase (ICL), malate synthase (MLS), and hydroxypyruvate reductase (HPR) are developmentally regulated. (A and B) Immunoblots of protein extracts from wild-type cotyledons (12 per lane) from 3- to 8-day-old seedlings were probed with antibodies to ICL, the cytosolic loading control HSC70, MLS, and HPR. Asterisk marks a cross-reacting band detected by the α-ICL antibodies. Positions of molecular weight markers (in kDa) are indicated on the right. (C) Photograph of light-grown 1- to 8-day-old wild-type (Col-0) seedlings. (D) Diagram of early postgerminative enzyme composition changes in Arabidopsis peroxisomes.