Fig. 5.

ICL is stabilized in pex5–10 and pex6–1. (A-C) Immunoblots of cotyledon (12 per lane) protein extracts from the indicated lines probed with α-ICL, α-MLS, α-PEX5, α-HPR, α-thiolase, and α-HSC70 antibodies. (D) Sucrose-dependent hypocotyl elongation of 35S-PEX5 lines. Seeds from lines in A-C were plated on the indicated medium and incubated overnight under white light, and were then incubated for 5 days in the dark. (E) IBA-resistant root growth of 35S-PEX5 lines. Seeds from A-C were plated on media containing 0.5% sucrose and the indicated IBA concentration and grown under yellow-filtered light for 7 days. (F) Immunoblot of protein extracts from whole sucrose-grown seedlings in D (eight 6-day-old dark-grown seedlings per lane) probed with α-PEX5 and α-HSC70 antibodies.