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. 2009 Feb 25;106(11):4183–4188. doi: 10.1073/pnas.0808880106

Fig. 2.

Fig. 2.

Characterization of SgcC5 as a condensation enzyme catalyzing both ester bond and amide bond formation. (A) SgcC5-catalyzed condensation reaction between SgcC2-tethered (S)-3-chloro-5-hydroxy-β-tyrosine and enediyne core mimics (R)-1-phenyl-1,2-ethanediol (1a), (R)-2-amino-1-phenyl-1-ethanol (2a), or (R)-2-phenylglycinol (3a) and the products established by spectroscopic analysis. HPLC profiles of time course analysis for SgcC5-catalzyed condensation between (S)-3-chloro-5-hydroxy-β-tyrosyl-(S)-SgcC2 incubated with (B) 1a and 1 μM SgcC5 for 1 min (i), 4 min (ii), 15 min (iii), and 90 min (iv), (C) 2a and 100 μM SgcC5 for 5 min (i), 15 min (ii), 60 min (iii), and 180 min (iv), and (D) 3a and 20 μM SgcC5 for 1 min (i), 2.5 min (ii), 5 min (iii), and 10 min (iv). 1b (◆), 1c (◇), 2b (▿), 3b (○), and 3c (●).