Thin-layer chromatography showing the production of farnesol from FPP by Phos15739. FPP (1 mM, 10 μL), FMP (1 mM, 10 μL), Farnesol (FOH, 1 mM, 10 uL), and Phos15739 (E, 400 ng in 10 μL) were used as standards. FPP and Phos15739 (E + FPP, 400ng and 10 μL of 1 mM respectively) were incubated at room temperature for 1 min or 30 min before loading. The mobile phase was hexane/ethyl acetate (4:1).