. Author manuscript; available in PMC: 2010 Feb 27.

Published in final edited form as: Biochem Biophys Res Commun. 2009 Jan 23;380(1):188–192. doi: 10.1016/j.bbrc.2009.01.079

Table 1.

Phosphatase activity of Phos15739 with isoprenoid mono- and pyrophosphate substrates

Substrate	Km (μM±S.E.)	Vmax (μmol.min⁻¹mg⁻¹±S.E.)	Kcat (s⁻¹±S.E.)	Kcat/Km (M⁻¹ s⁻¹)
IMP	84 ± 27	18 ± 2.7	10 ± 1.5	1.2 × 10⁵
GMP	81 ± 6.7	250 ± 9.3	140 ± 5.3	18 × 10⁵
FMP	160 ± 20	85 ± 4.2	49 ± 2.4	3.1 × 10⁵
GGMP^a	>1000^a	ND^a	ND^a	ND^a
IPP^b	430 ± 150	2.1 ± 0.39	1.2 ± 0.22	0.027 × 10⁵
GPP^b	20 ± 8. 3	1.4 ± 1.5	0.78 ±0.09	250.39 × 10⁵
FPP^b	48 ± 13	18 ± 1.8	10 ± 1.04	2. 1 × 10⁵
GGPP^a	>1000^a	ND^a	ND^a	ND^a

GGMP and GGPP are poor substrates of Phos15739, kinetics could not be determined (ND)

Values are reported as apparent kinetics.