Fig. 2.
Experimental protocols for cell viability assay in ischemia/reperfusion injury. Using an established chick cardiomyocyte model of simulated ischemia/reperfusion, tested flavonoids were: incubated since 72 h before ischemia and continued through ischemia/reperfusion (chronic treatment), treated during ischemia and reperfusion (ischemia/reperfusion treatment), or administered at reperfusion only (reperfusion treatment).