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. 2009 Feb 9;60(4):1273–1288. doi: 10.1093/jxb/erp008

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© 2009 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 5. — Changes in the main free (A) and esterified (B) oxylipins during ageing. The fatty acid hydroperoxides (HPOs) and hydroxides (HOs) derived from linoleic acid (‘D’ derivatives) were measured according to Göbel et al. (2002) and Fauconnier et al. (2008) using a two-step HPLC method combining reverse and normal phase analysis. Two independent extracts were used for each point of the kinetics. The change in carbonyl content (C) was measured spectrophotometrically after SDS extraction and DNPH derivatization. The presented data are means of three independent replicates ±SD. Means sharing the same letter were not statistically distinct using the Newman and Keuls test. The non-significant (N.S.) changes are mentioned on the graphs.