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. 2009 Feb 26;60(4):1375–1386. doi: 10.1093/jxb/erp020

Fig. 2.

Fig. 2.

Nuclear DNA fragmentation detected by TUNEL assay in Scots pine seed sections. (A) TUNEL-positive signals in the megagametophyte in the vicinity of the corrosion cavity at the early embryogeny stage. (B) TUNEL-positive signals in the megagametophyte in the arrow-shaped region in front of the embryo at the late embryogeny stage. (C) Slightly TUNEL-positive nuclei appeared in the inner part of the megagametophyte during the late embryogeny stage (sampling date IV). (D) TUNEL-positive nuclei in the nucellar layer at the late embryogeny stage. (E) TUNEL-positive nuclei in the suspensor cells at the early embryogeny stage. (F) TUNEL-positive nuclei detected by excitation at 543 nm in the inner part of the megagametophyte at the late embryogeny stage. (G) Lack of DNA fragmentation detected by excitation at 543 nm in the inner part of the megagametophyte at the early embryogeny stage. (H) Positive control (DNase treatment). (I) Negative control (omission of TdT). Bars: (C, D, F) 20 μm, (B, E, G, H, I) 50 μm, and (A) 100 μm.