Fig. 4.

The expression of the putative RAD51, KU80, LIG, MCA, and TAT-D genes in developing Scots pine seeds. The expressions were quantified by real-time PCR and normalized by the expression derived from the housekeeping genes ACT, UBI, and GAPDH. The observed relative gene expressions are presented in relation to the effective temperature sum (d.d.) in the clones K818 (open circles) and K884 (closed circles), and the geometric mean values of the replicates pertaining to the same clone and sampling date are connected by dashed lines for K881 and solid lines for K884. There are five replicates per clone and per sampling date, except for the seeds of clone K884 on sampling dates III and IV, from which there are two and four replicates, respectively.