Fig. 1.

Amino acid sequence alignment and phylogenetic tree of HT-like proteins. (A) The amino acid sequences of HT-like proteins were aligned by CLUSTAL W (Thompson et al., 1994). Putative signal peptides are shown in lower case. A chacteristic deletion for HT-A is denoted with #. The N/D-rich domain was boxed. (B) A Neighbor–Joining tree (Saitou and Nei, 1987) of HT-like proteins. NOD24 protein of soybean (GmNOD24; M10595) was chosen as an outgroup for rooting, because it is a member of the recently proposed expanded HT family, the HT/NOD-24 family, and is distantly related to solanaceous HT-like proteins (Kondo and McClure, 2008). Numbers denote bootstrap values with 1000 replicates.