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RNA blot analysis of PiHT-B and PhHT-B in styles of P. inflata, ‘Mitchell’ and P. axillaris subsp. parodii. A 10 μg aliquot of total RNA was loaded in each lane, blotted, and hybridized with P. inflata (A, PiHT-B) and ‘Mitchell’ HT-B (B, PhHT-B) probes. Aliquots of 1 μg and 0.1 μg of P. inflata RNA were also loaded in separate lanes to allow for comparison of the level of expression. The ethidium bromide-stained gels are shown to ascertain equal loading conditions.
