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Transgenic plant experiments testing the role of PiHT-B in the S-specific pollen rejection. The RNAi construct for PiHT-B was first introduced into the SC line ‘Mitchell’. The transgenics were crossed with SI lines P. inflata (S3LS3L, HT-BiHT-Bi) or NIL Mitchell (S3LS3L, HT-BiHT-Bi) to produce ‘hybrid background’ or ‘Mitchell background’ transgenics, respectively, and used for analysis of the S-specific pollen rejection.
