Fig. 5.

RNA blot analysis of PiHT-B in styles of RNAi transgenic plants. (A) Hybrid background transgenic lines 78, 87, 96, 176, and 199 [transgenic ‘Mitchell’×P. inflata (S^3LS^3L)]. (B) Mitchell background transgenic lines 8, 52, 157, 173, and 208 [transgenic ‘Mitchell’×NIL Mitchell (S^3LS^3L, HT-BⁱHT-Bⁱ)]. A 10 μg aliquot of pistil total RNA was loaded in each lane. Aliquots of 1 μg and 0.1 μg of RNA of the untransformed plants were also loaded in separate lanes to assess the level of suppression. CH and CM are untransformed controls of hybrid and Mitchell backgrounds, respectively. The ethidium bromide-stained gel is shown to ascertain equal loading conditions.