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RNA blot analysis of S3L-RNase in styles of RNAi lines for HT-B. Severely HT-B-suppressed Mitchell background plants 52 and 208 were analysed. ‘Mitchell’ and untransformed Mitchell background (CM) are negative and positive controls, respectively. A 10 μg aliquot of style total RNA was loaded in each lane, blotted, and hybridized with the P. inflata S3L-RNase probe. The ethidium bromide-stained gel is shown to ascertain equal loading conditions.
