Fig. 2.

Tissue-specific and ethylene-dependent expression of Sl-IAA3. The expression analyses were carried out by qRT-PCR using RNA samples extracted from various tomato tissues. (A) Analysis of Sl-IAA3 transcript levels in different organs. SI-IAA3 mRNA accumulation was monitored in stem (S), leaf (L), flower (F), root (R), and red fruit (Re). (B) Expression pattern of SI-IAA3 during the late stages of fruit development: immature green fruit, IMG; mature green, MG; breaker, Br; turning, Tu; orange, Or; red, Re; red-ripe, RR. (C) Expression pattern of Sl-IAA3 in wild type (WT) and rin, nor, and Nr ripening mutants. RNA samples were extracted from fruit collected 43 d and 70 d after anthesis, corresponding in the WT to MG and Re stages, respectively. (D) Ethylene responsiveness of the Sl-IAA3 gene. RNA samples were extracted from MG fruit treated for 5 h with air or with 50 μl l⁻¹ ethylene. (E) Br fruit treated with 1 μl l⁻¹ of 1-MCP for 16 h. Relative expression level on the y-axis refers to the fold difference in Sl-IAA3 expression relative to stem in (A), MG stage in (B, C), and untreated control fruit in (D, E). The expression data are means of three replicates ±standard error.