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. 2009 Jan 22;28(5):591–601. doi: 10.1038/emboj.2009.4

Figure 2.

Figure 2

UVR8 protein amount is rate limiting for UV-B-induced photomorphogenesis. (A) Quantitative RT–PCR data showing overexpression of UVR8 but no effect on COP1 expression in lines Ox nos. 2 and 3 compared with wild type. (B) Hypocotyl length measurements of 4-day-old seedlings grown with or without supplemental UV-B. Error bars represent s.d. (n=30). (C) Luciferase assays visualizing HY5 promoter activation in response to UV-B in UVR8 overexpression lines nos. 2 and 3 compared with wild type. Error bars represent s.e. (n=30). (D, E) Quantitative RT–PCR analysis of HY5 and CHS gene activation in response to UV-B in UVR8 overexpressor lines compared with wild type. (F) Immunoblot analysis of UVR8, CHS and actin (loading control) protein levels in 4-day-old seedlings grown with or without supplementary UV-B. (G) Anthocyanin accumulation of 4-day-old seedlings grown with or without supplemental UV-B. Error bars represent s.d. (n=3). (A–G) WT=Ws/ProHY5:Luc; Ox no. 2/no. 3=Pro35S:UVR8 in WT, lines 2 and 3.