Table 1. Characteristics of 4 VNTR loci used for identification of Burkholderia pseudomallei*.
| Characteristic | VNTR loci |
|||
|---|---|---|---|---|
| 2341 | 1788 | 933 | 389 | |
| Color-labeled forward primer sequence (5′ → 3′) | FAMGGCTTCGCACCCGCCCCATTTCAGC | PETGCGCGGCGAGAACGGCAAGAACGAA | NEDATGGTGGCGGCCGTCGGCGAAAACC | VICGTTACAAGCGCGGGTCGGCAAGAGGCTGAAA |
| Reverse primer sequence (5′ → 3′) | GCACCGGGCGCGGCGCACTCG | GAGCATCGGGTGGGCGGCGCGTATTGAT | GCTCGAATGGGTGTACGAAGGGCCACGCTGATTC | GCCGGTGTTGAACGAGTGGGTGGCGTAAGC |
| Repeat sequence (5′ → 3′) | TTCGTGCGC | GTCGTGCGATCCTGCT | CGGCGAGGGAAA | GACGAACC |
| Minimum size, bp† (no. repeats) | 111 (2) | 235 (4) | 171 (3) | 221 (1) |
| Maximum size, bp (no. repeats) | 243 (17) | 382 (13) | 337 (17) | 292 (10) |
| No. alleles | 16 | 10 | 13 | 9 |
| No. null alleles | – | – | 2/65 STs | – |
*VNTR, variable number tandem repeat; STs, sequence types. †Error range in fragment sizing is ± 3 bp.