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TM10 binds to the surface of tumour but not to untransformed cells, and there is a large intracellular reserve of its epitope in all cells. (A) Immunofluorescent microscopy (objective x63) of B16F10 cells surface stained and intracellular stained (permeabilised with Triton X-100) with TM10 or isotype control. Bar = 10μm. (B) Surface and intracellular (permeabilised with saponin) staining of human PBLs with TM10. Closed line - isotype control, open line = mAb staining.
