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. Author manuscript; available in PMC: 2009 Mar 18.
Published in final edited form as: Biochemistry. 2008 Jan 9;47(5):1381–1392. doi: 10.1021/bi701189c

Figure 2.

Figure 2

All three PD-associated mutations thermally destabilize DJ-1. Differential scanning calorimetry (DSC) of wtDJ-1 (filled circles), M26I (open squares), A104T (filled squares) and E163K (grey triangles) shows that each pathogenic mutation reduces the apparent melting temperature (Tm) of DJ-1. The molar heat capacity at constant pressure (Cp) is calculated from the observed endotherms (ordinate), but cannot be subjected to thermodynamic analysis due to the irreversibility of DJ-1 denaturation. The Tm for each sample reported in the text is taken from the fit of a non-two state unfolding transition to the measured endotherm.