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The secondary structural content of DJ-1 in solution is unaffected by three PD-associated mutations. Circular dichroism (CD) spectra were collected at 20°C, pH=7.5 for 10μM wtDJ-1 (filled circles), M26I (open squares), A104T (filled squares) and E163K (grey triangles) and show no significant differences in the mean residue molar ellipicity ([Θ]; ordinate) as a function of wavelength.
