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. Author manuscript; available in PMC: 2009 Mar 18.
Published in final edited form as: Biochemistry. 2008 Jan 9;47(5):1381–1392. doi: 10.1021/bi701189c

Figure 6.

Figure 6

Hydrogen bonding stabilizes discrete disorder at T104 in A104T DJ-1. Panel A shows the environment of an engineered A104V substitution in DJ-1. Inspection of 1.85 Å resolution 2mFo-DFc electron density at 1.0σ (blue) shows that V104 is less disordered than T104, despite the steric similarity of these two residues. Positive difference (mFo-DFc) electron density contoured at 3.0σ (green), however, indicates that the residue likely samples a second minor conformation. In panel B, T104 makes two mutually exclusive hydrogen bonds that favor discrete disorder for this residue. The higher occupancy conformation for T104 is shown in the darker line, electron density (2mFo-DFc) is contoured at 1.0σ (blue), and hydrogen bonds are shown in dashed lines with their distances provided in Ångstroms. The orientation of residue 104 in panels A and B is the same. The figure was created with POVscript+(65).