Table 1.

Strain construction

ID primer	Gene	Direction	Sequence
1	pcbC	FWD	GAT TGG CGC TCC TCG TTC ACC
2	pcbC	REV	CCA TTA TTT TTC TAG TCG ACA TGG CAT CGA TTC CCA AGG CCA ATG TCC CC
5	LF 7-kb	FWD	GTT ACA CGC TTT GAT TCT GTG GGT ACC GAT GTT ATA TTC AGC TAC
6	LF	REV	CCC AAT AGC GGC CGC AGT TGA TAA TAT CAA TAT CTA AAA CTC CC
7	LF 5-kb	FWD	GGC ATA TAC GAG CAT GGT ACC AGG GAC AGA TGC CCA TCC TTG
8	LF 3-kb	FWD	GTA TAA AAG GGG AGG GTA CCG GGA AAG ATT TGT GGG CCT G
9	RF	FWD	GTA TGT AGC TGC GGC CGC CTC CGT CTT CAC TTC TTC GCC CGC ACT
10	RF 3-kb	REV	CCG CCT TCC TCA CTA ACC GGC CGG CAG GTA CCG ATG GAC TCA GCA TTA TC
11	RF 5-kb	REV	CTC TAG AAT GCT ACG GCC GTT CGA GGT ACC TTA TAG GAA AAA GGT AG
12	RF 7-kb	REV	CCT TTT CGC TGA GCG GCC GCA ATC ACA GGT ACC GTT TTT GTC GTC
13	amdS	FWD	ATG CCT CAA TCC TGG GAA GAA CTG
14	amdS	REV	CTT GAC GTA GAA GAC GGC ACC GGC
15	penDE	FWD	CCC GCA GCA CAT ATG CTT CAC ATC CTC TGT CAA GGC
16	penDE	REV	ATG ACA AAC ATC TCA TCA GGG
17	niaA	FWD	CAC AGA GAA TGT GCC GTT TCT TTG G
18	niaA	REV	TCA CAT ATC CCC TAC TCC CGA GCC
19	pcbAB	FWD	GAA GAC GTC ATA CTT ATT CTC TG
20	pcbAB	REV	CGG CAT CGG ATA AAG AGA TCT GG

Primers used in this study (restriction sites are in bold). LF and RF, Left and Right flanking region respectively, location of the flanking region relative to the amdS-cassette (see Figure 1A). Size of the flanking region in kb. ID primer, uniquely numbered identifier of the primer used.