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. 2009 Feb 10;10:75. doi: 10.1186/1471-2164-10-75

Table 2.

Physiological and microarray quality parameters for aerobic, glucose-limited chemostat cultures grown at a dilution rate of 0.03 h-1

DS17690 - PAA DS17690 + PAA DS50661 - PAA DS50661 + PAA
Ysxa (g·g-1) 0.37 ± 0.01 0.35 ± 0.01 0.39 ± 0.01 0.36 ± 0.01
qpenb (μmol·g-1·h-1) 0.00 ± 0.00 19.81 ± 1.47 0.00 ± 0.00 0.00 ± 0.00
qPAAc (μmol·g-1·h-1) 0.00 ± 0.00 24.04 ± 2.38 0.00 ± 0.00 5.60 ± 0.38
qCO2d (mmol·g-1·h-1) 1.15 ± 0.08 1.42 ± 0.11 1.16 ± 0.05 1.44 ± 0.10
qO2e (mmol·g-1·h-1) 1.19 ± 0.11 1.42 ± 0.17 1.16 ± 0.11 1.43 ± 0.08
Avg CVf 0.21 0.18 0.17 0.14
Pc actAg 4190 ± 170 3560 ± 360 3450 ± 390 3030 ± 680
Pc gdh2h 1240 ± 120 1140 ± 270 1020 ± 170 1060 ± 180
Repeats (n) 3 4 3 3

P. chrysogenum strains DS17690 (penicillinG high producing) and DS50661 (lacking a functional penicillin gene cluster) were grown in the presence and absence of phenylacetic acid (PAA) in independent glucose-chemostat cultures at D = 0.03 h-1. Results are the averages ± S.D. (σn-1).

a biomass yield on glucose (g of biomass per g of glucose consumed)

b biomass specific penicillinG production rate. In addition to penicillinG, intermediates and byproducts are formed, which accounts for ~6% of the consumed PAA [30] (μmol of penicillin G produced per g of dry weight biomass and per · hour)

c Biomass specific phenylacetate consumption rate (μmol of phenylacetate consumed per g of dry weight biomass and per hour)

d Biomass specific CO2 production rate (mmol of CO2 produced per g of dry weight biomass and per hour)

e Biomass specific O2 consumption rate (mmol of O2 consumed per g of dry weight biomass and per hour)

f the average coefficient of variation (standard deviation divided by the mean) for all genes except the genes with mean below 12

g encoding actin; average hybridization signal and standard deviation

h encoding glutamate dehydrogenase (NAD+ dependent); average hybridization signal and standard deviation